The sale is part of RailAmerica's plan to sell non-core, non-strategic assets and reduce debt.
Boca Raton, Fla.-based RailAmerica has suspended regular freight service on the rail line, which runs between Wayzata and Hutchinson, Minn., for more than a year, due to insufficient shipper demand.
The company owns 39 short-line and regional railroad, operating 11,000 route miles in 22 states and six Canadian provinces, as well as Australia and Chile.
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